• Fluorometric and fluorescence-based assays emerged as the dominant analytical approach for sensitive measurement of catecholamines and related amines in tissues and blood, evolving from adrenaline/noradrenaline fluorimetric methods to dopamine and histamine assays with improvements in selectivity and speed. [3], [6], [7], [8], [9], [10], [11], [12], [18]

• Chromatographic separation methods (gas-liquid chromatography and paper chromatography) enabled quantitative monitoring of anticonvulsants and steroids in biological samples, underpinning clinical drug testing and pharmacokinetic studies. [1], [2], [5], [15], [20]

• A spectrum of fluorometric strategies targeted adrenaline, noradrenaline, dopamine, histamine and related amines in plasma and tissues, employing hydroxyindole and micromethod approaches to achieve sensitive, specific assays. [3], [7], [8], [9], [11], [12], [14], [18]

• Analytical focus extended to pharmacokinetics, with studies on drug-protein binding and absorption/distribution shaping analytical strategies for monitoring drug levels and disposition. [14], [16], [17]

• Rapid clinical toxicology and therapeutic monitoring featured GC-based assays for diphenylhydantoin and derivatives, enabling timely drug testing in serum/plasma. [1], [2], [5], [15]